Vitamin D Receptor anticorps (AA 89-105)

N° du produit ABIN962160

Cet anticorps anti-Vitamin D Receptor Monoclonal Rat (ABIN962160) détecte spécifiquement Vitamin D Receptor dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Vitamin D Receptor anticorps (AA 89-105) (ABIN962160)

Antigène: Vitamin D Receptor (VDR)

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Vitamin D Receptor est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Vitamin D Receptor anticorps

Épitope: AA 89-105

Specificité: Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.

Purification: Protein G purified

Immunogène: Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.

Type of Immunogen: Purified protein

Isotype: IgG2b

Information d'application

Indications d'application: Approved: IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: LNCaP and HeLa cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide. Sourced in Ascites.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Détails sur Vitamin D Receptor

Antigène: Vitamin D Receptor (VDR)

Autre désignation: Vitamin D Receptor / VDR

Classe de substances: Chemical

Sujet: Name/Gene ID: VDR
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: VDR, NR1I1, Vitamin D3 receptor, Vitamin D receptor

ID gène: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway