His Tag anticorps

N° du produit ABIN964552

N° du produit (Fournisseur): 200-301-382

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, ELISA et IP. Il présente une réactivité envers et a été mentionné dans 4+ publications.

Aperçu rapide pour His Tag anticorps (ABIN964552)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 33D10-D2-G8

Détail du produit anti-His Tag anticorps

N° du produit (Fournisseur): 200-301-382

Fournisseur: Rockland

Fonction: 6X His Epitope Tag Antibody

Séquence: HHHHHH

Réactivité croisée (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Attributs du produit: Synonyms: mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Purification: 6X HIS Epitope Tag antibody was purified from concentrated tissue culture supernate by Protein A chromatography and is directed against the 6X His motif and is useful in determining its presence in various assays.

Stérilité: Sterile filtered

Immunogène:

Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Informations sur le produit:

À quoi peut servir l'anticorps His-tag ABIN964552 ? Cet anticorps His-tag est un anticorps monoclonal non-conjugué pour la détection spécifique des protéines de fusion contenant des tags épitopes His amino- ou carboxy-terminaux. Il convient pour la détection de His-tag dans les expériences ELISA et Western blot, IHC et IP.

Quelles sont les données de validation disponibles pour cet anticorps His-tag ? L'anticorps His-tag a été utilisé dans 4 publications à ce jour, qui peuvent être consultées sur Pubmed. Quatre images sont disponibles pour la validation de l'anticorps en western blotting et en ELISA.

Quelle est la fonction de His-Tag ? Le His-tag est l'un des tags les plus couramment utilisés. Il s'agit d'une série de six à neuf résidus histidine fusionnés à l'extrémité amino ou carboxyle d'une protéine recombinante. Le marquage His est particulièrement utile car il permet une purification et une détection faciles de la protéine cible. La petite taille du marqueur His par rapport à d'autres marqueurs d'épitope courants le rend moins susceptible d'interférer avec la structure ou la fonction de la protéine cible. Par conséquent, il est bien adapté à une utilisation dans des conditions de dénaturation. Utilisez notre anticorps His-tag de haute qualité pour détecter de manière fiable le His-tag.

Information d'application

Indications d'application:

Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, Anti-6X His/6XHIS can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Western Blot Dilution: 1:1000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:75,000

Other: FLUORESCENT POLARIZATION: 2 - 20 nMFRET: 2 - 20 nM

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 3.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store 6X HIS Tag Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-6X HIS Tag is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-His Tag (ABIN964552)

Zhou, Pu, Chen, Hao, Gao, Ali, Hsiao, Stock, Goulian, Zhu: "Thiol-based functional mimicry of phosphorylation of the two-component system response regulator ArcA promotes pathogenesis in enteric pathogens." dans: Cell reports, Vol. 37, Issue 12, pp. 110147, (2022) (PubMed).

Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." dans: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

Chavda, Ling, Majernick, Planey: "Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain." dans: BMC biochemistry, Vol. 18, Issue 1, pp. 13, (2017) (PubMed).

Ramón-García, Ng, Jensen, Dosanjh, Burian, Morris, Folcher, Eltis, Grzesiek, Nguyen, Thompson: "WhiB7, an Fe-S-dependent transcription factor that activates species-specific repertoires of drug resistance determinants in actinobacteria." dans: The Journal of biological chemistry, Vol. 288, Issue 48, pp. 34514-28, (2014) (PubMed).

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6X His

Classe de substances: Tag

Sujet: Background: 6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.