His Tag anticorps (HRP)

N° du produit ABIN964554

N° du produit (Fournisseur): 200-303-382

Cet anticorps anti-His Tag est un anticorps Souris Monoclonal détectant His Tag dans WB, ELISA et IHC. Adapté pour . Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour His Tag anticorps (HRP) (ABIN964554)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 33D10-D2-G8

Détail du produit anti-His Tag anticorps (HRP)

N° du produit (Fournisseur): 200-303-382

Fournisseur: Rockland

Fonction: 6X His Tag Antibody HRP Conjugated

Séquence: HHHHHH

Réactivité croisée (Details): This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag and is useful in determining its presence in various assays.

Attributs du produit: Synonyms: mouse anti-6X His Tag HRP conjugated Antibody, peroxidase conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

Purification: 6X His antibody is directed against the 6X His motif and was purified from concentrated tissue culture supernate by Protein A chromatography.

Immunogène:

Immunogen: This 6X his antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-His Tag (HRP) (ABIN964554)

Li, Xu, Wang, Gong, Liu, Zheng, Li, Li: "Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1." dans: mAbs, Vol. 9, Issue 4, pp. 628-637, (2018) (PubMed).

Détails sur His Tag

Antigène: His Tag

Autre désignation: 6X His

Classe de substances: Tag

Sujet: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.