ATG13 anticorps

N° du produit ABIN964567

N° du produit (Fournisseur): 600-401-c50

Cet anticorps Lapin Polyclonal détecte spécifiquement ATG13 dans WB et ELISA. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour ATG13 anticorps (ABIN964567)

Antigène: ATG13 (Autophagy Related 13 (ATG13))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG13 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ATG13 anticorps

N° du produit (Fournisseur): 600-401-c50

Fournisseur: Rockland

Fonction: ATG13 Antibody

Réactivité croisée (Details): This affinity-purified antibody is directed against human ATG13 protein.

Attributs du produit: Synonyms: rabbit anti-ATG13 Antibody, ATG-13, ATG 13, Autophagy-related protein 13, KIAA0652

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 56.6 kDa in size corresponding to human ATG13 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract. 

Western Blot Dilution: 1:1000

ELISA Dilution: 1:220,000-1:250,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.24 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-ATG13 (ABIN964567)

Stanton, Dutta, Zhang, Polavaram, Leontovich, Hönscheid, Sinicrope, Tindall, Muders, Datta: "Autophagy control by the VEGF-C/NRP-2 axis in cancer and its implication for treatment resistance." dans: Cancer research, Vol. 73, Issue 1, pp. 160-71, (2013) (PubMed).

Nazio, Strappazzon, Antonioli, Bielli, Cianfanelli, Bordi, Gretzmeier, Dengjel, Piacentini, Fimia, Cecconi: "mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6." dans: Nature cell biology, Vol. 15, Issue 4, pp. 406-16, (2013) (PubMed).

Joo, Dorsey, Joshi, Hennessy-Walters, Rose, McCastlain, Zhang, Iyengar, Jung, Suen, Steeves, Yang, Prater, Kim, Thompson, Youle, Ney, Cleveland, Kundu: "Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy." dans: Molecular cell, Vol. 43, Issue 4, pp. 572-85, (2011) (PubMed).

Détails sur ATG13

Antigène: ATG13 (Autophagy Related 13 (ATG13))

Autre désignation: ATG13

Sujet: Background: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.

ID gène: 9776

NCBI Accession: NP_001136145

UniProt: O75143

Pathways: Signalisation PI3K-Akt, Autophagy