beta Actin anticorps (AA 350-375) (HRP)

N° du produit ABIN964577

N° du produit (Fournisseur): 600-403-886

L’anticorps Lapin Polyclonal anti-beta Actin a été validé pour WB, ELISA et IHC. Il convient pour détecter beta Actin dans des échantillons de Humain, Souris, Rat, Singe, Porc, Boeuf (Vache), Mouton, Hamster, Cobaye et Chévre. Il y a 1 publication disponible.

Aperçu rapide pour beta Actin anticorps (AA 350-375) (HRP) (ABIN964577)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Souris, Rat, Singe, Porc, Boeuf (Vache), Mouton, Hamster, Cobaye, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-beta Actin anticorps (HRP)

Épitope: AA 350-375

N° du produit (Fournisseur): 600-403-886

Fournisseur: Rockland

Fonction: Beta Actin Antibody Peroxidase Conjugated

Réactivité croisée (Details): Beta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein.

Attributs du produit: Synonyms: rabbit anti-beta Actin Antibody HRP conjugation, peroxidase conjugated rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogène: Optional[Immunogen]: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Beta Actin Antibody Peroxidase Conjugated antibody has been tested for use in ELISA and is suitable in immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly.  Beta actin present in neuromuscular junctions also stains.  Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation.  Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-beta Actin (HRP) (ABIN964577)

Xie, Collins: "Copper stabilizes the Menkes copper-transporting ATPase (Atp7a) protein expressed in rat intestinal epithelial cells." dans: American journal of physiology. Cell physiology, Vol. 304, Issue 3, pp. C257-62, (2013) (PubMed).

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: ACTB

Sujet: Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility.  Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Peroxidase conjugate Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction