DYKDDDDK Tag anticorps (FITC)

N° du produit ABIN964626

N° du produit (Fournisseur): 200-302-383

Cet anticorps anti-DYKDDDDK Tag est un anticorps Souris Monoclonal détectant DYKDDDDK Tag dans WB, ELISA et FM. Adapté pour .

Aperçu rapide pour DYKDDDDK Tag anticorps (FITC) (ABIN964626)

Antigène: DYKDDDDK Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYKDDDDK Tag est conjugé à/à la FITC

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Clone: 29E4-G7

Détail du produit anti-DYKDDDDK Tag anticorps (FITC)

N° du produit (Fournisseur): 200-302-383

Fournisseur: Rockland

Fonction: DYKDDDDK Tag (Anti-FLAG®) Antibody Fluorescein Conjugated

Séquence: DYKDDDDK

Réactivité croisée (Details): This antibody is directed against the FLAG epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Attributs du produit: Synonyms: mouse anti-FLAG™ tag FITC conjugation, Enterokinase Cleavage Site (ECS), Fluorescein conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Immunogène:

Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG2a kappa

Labeling Ratio: 3.1

Information d'application

Indications d'application:

Application Note: Anti-FLAG Fluorescein has been tested in ELISA and western blot. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Autre désignation: FLAG

Classe de substances: Tag

Sujet: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.