LYZ anticorps (AA 1-129)

N° du produit ABIN964704

N° du produit (Fournisseur): 200-401-072

Cet anticorps anti-LYZ est un anticorps Lapin Polyclonal détectant LYZ dans WB, IHC, IP, ELISA et DB. Adapté pour Poulet.

Aperçu rapide pour LYZ anticorps (AA 1-129) (ABIN964704)

Antigène: LYZ (Lysozyme (LYZ))

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LYZ est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Dot Blot (DB)

Détail du produit anti-LYZ anticorps

Épitope: AA 1-129

N° du produit (Fournisseur): 200-401-072

Fournisseur: Rockland

Fonction: Lysozyme Antibody

Réactivité croisée (Details): This purified antibody is directed against lysozyme from hen egg white protein.

Attributs du produit: Synonyms: rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length protein corresponding to amino acids 1-129 of Hen Egg White Lysozyme.

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Lysozyme Hen Egg White purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~14 kDa in size corresponding to lysozyme by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/ml

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur LYZ

Antigène: LYZ (Lysozyme (LYZ))

Autre désignation: LYZ

Sujet: Background: Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of β-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers.  Lysozyme is common in animals and plants.  In birds, lysozyme is also an exceptionally abundant protein in egg whites.  Its biological function in fowl eggs is unclear.  Hen egg white lysozyme ''c'' is the most commonly studied form and source of the enzyme.  Lysozyme from domestic goose is designated lysozyme ''g''.  Lysozyme is also found in vertebrates, including human, mostly in secretions and certain tissues, such as saliva, tears, milk, cervical mucus, leucocytes, kidneys and urine, where it probably serves as an anti-bacterial agent by digesting and weakening the rigid bacterial cell wall, thereby rendering the bacteria susceptible to osmotic lysis.  Plant lysozyme is found in ficus and papaya latex, and is chemically distinct from the egg white enzyme.

ID gène: 396218

NCBI Accession: NP_990612

UniProt: P00698