IL-7 anticorps (Biotin)

N° du produit ABIN964779

N° du produit (Fournisseur): 209-406-b94

Cet anticorps anti-IL-7 est un anticorps Lapin Polyclonal détectant IL-7 dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour IL-7 anticorps (Biotin) (ABIN964779)

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-7 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-IL-7 anticorps (Biotin)

N° du produit (Fournisseur): 209-406-b94

Fournisseur: Rockland

Fonction: IL-7 Antibody Biotin Conjugated

Attributs du produit: Synonyms: rabbit anti-IL-7 biotin conjugated antibody, rabbit anti-interleukin-7 antibody, IL7, IL-7 Interleukin 7, Interleukin-7

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogène:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000-1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur IL-7

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: IL7

Sujet: Background: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.

ID gène: 3574

NCBI Accession: NP_000871

UniProt: P13232

Pathways: Signalistation JAK/STAT