GAPDH anticorps (N-Term)

N° du produit ABIN966181

Cet anticorps Lapin Polyclonal détecte spécifiquement GAPDH dans WB, ICC, IHC (p) et IHC (f). Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 2+ publications.

Aperçu rapide pour GAPDH anticorps (N-Term) (ABIN966181)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Détail du produit anti-GAPDH anticorps

Épitope: N-Term

Réactivité croisée: Souris, Rat (Rattus)

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminal of human GAPDH , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.

Isotype: IgG

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Références pour anticorps anti-GAPDH (ABIN966181)

Colell, Ricci, Tait, Milasta, Maurer, Bouchier-Hayes, Fitzgerald, Guio-Carrion, Waterhouse, Li, Mari, Barbry, Newmeyer, Beere, Green: "GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation." dans: Cell, Vol. 129, Issue 5, pp. 983-97, (2007) (PubMed).

Benham, Povey: "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations." dans: Genomics, Vol. 5, Issue 2, pp. 209-14, (1989) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: Glyceraldehyde-3-phosphate dehydrogenase(GAPDH)

Sujet: Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme is thought to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase (GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.