NUMA1 anticorps (Cytoplasmic, Nuclear)

N° du produit ABIN966706

Cet anticorps Souris Monoclonal détecte spécifiquement NUMA1 dans ELISA, IHC (p) et IHC (fro). Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour NUMA1 anticorps (Cytoplasmic, Nuclear) (ABIN966706)

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NUMA1 est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3-3A

Détail du produit anti-NUMA1 anticorps

Épitope: Cytoplasmic, Nuclear

Specificité: Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.

Purification: Concentrated.

Immunogène: Live Ls 174T cells (colon carcinoma).

Isotype: IgM

Information d'application

Indications d'application: Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Commentaires:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-NUMA1 (ABIN966706)

Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." dans: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).

Détails sur NUMA1

Antigène: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Autre désignation: NuMA

Poids moléculaire: 228 kDa

ID gène: 4926

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase