NUMA1 anticorps (Nuclear Mitotic Apparatus Protein 1) (Cytoplasmic)

Details for Product anti-NUMA1 Antibody No. ABIN966706, Fournisseur: Connectez-vous pour afficher
Antigène
  • NUMA
  • 6720401E04Rik
  • AA764025
  • AL022610
  • AU014979
  • NUMA1
  • NuMA
  • SP-H
  • numa
  • numa1
  • nuclear mitotic apparatus protein 1
  • nuclear mitotic apparatus protein 1 S homeolog
  • NUMA1
  • Numa1
  • numa1
  • numa1.S
Épitope
Cytoplasmic, Nuclear
15
6
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivité
Humain
121
29
27
3
3
2
2
1
1
1
1
1
1
1
1
Hôte
Souris
79
43
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp NUMA1 est non-conjugé
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
57
45
31
30
26
26
26
22
13
8
3
2
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Live Ls 174T cells (colon carcinoma).
Clone 3-3A
Isotype IgM
Specificité Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
Purification Concentrated.
Plasmids, Primers & others Plasmids, Primers & others NUMA1 products on genomics-online (e.g. as negative or positive controls)
Autre désignation NuMA (NUMA1 Antibody Extrait)
Poids moléculaire 228 kDa
ID gène 4926
Pathways Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
Indications d'application Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Commentaires

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Produit citée dans: Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." dans: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, 1997 (PubMed).

Background publications Butschak, Harborth, Osborn, Karsten: "New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells." dans: Acta histochemica, Vol. 97, Issue 1, pp. 19-31, 1995 (PubMed).

Compton, Szilak, Cleveland: "Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis." dans: The Journal of cell biology, Vol. 116, Issue 6, pp. 1395-408, 1992 (PubMed).

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