NUMA1 anticorps (Nuclear Mitotic Apparatus Protein 1) (Cytoplasmic)

Details for Product anti-NUMA1 Antibody No. ABIN966706, Fournisseur: Connectez-vous pour afficher
Antigène
  • NUMA
  • 6720401E04Rik
  • AA764025
  • AL022610
  • AU014979
  • NUMA1
  • NuMA
  • SP-H
  • numa
  • numa1
  • nuclear mitotic apparatus protein 1
  • nuclear mitotic apparatus protein 1 S homeolog
  • NUMA1
  • Numa1
  • numa1
  • numa1.S
Épitope
Cytoplasmic, Nuclear
15
6
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Reactivité
Humain
102
30
28
3
3
3
3
2
2
1
1
1
1
1
1
Hôte
Souris
77
26
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp NUMA1 est non-conjugé
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
56
32
29
29
26
23
13
8
7
3
2
1
1
1
1
Options
Fournisseur
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N° du produit (Fournisseur)
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Immunogène Live Ls 174T cells (colon carcinoma).
Clone 3-3A
Isotype IgM
Specificité Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
Purification Concentrated.
Autre désignation NuMA (NUMA1 Antibody Extrait)
Poids moléculaire 228 kDa
ID gène 4926
Pathways Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase
Indications d'application Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Commentaires

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C
Produit citée dans: Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." dans: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, 1997 (PubMed).

Background publications Butschak, Harborth, Osborn, Karsten: "New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells." dans: Acta histochemica, Vol. 97, Issue 1, pp. 19-31, 1995 (PubMed).

Compton, Szilak, Cleveland: "Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis." dans: The Journal of cell biology, Vol. 116, Issue 6, pp. 1395-408, 1992 (PubMed).

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