MED23 anticorps

N° du produit ABIN967286

Détail du produit anti-MED23 anticorps

Antigène: MED23 (Mediator Complex Subunit 23 (MED23))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MED23 est non-conjugé

Application: Western Blotting (WB)

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Sur-2 synthetic peptide

Clone: D27-1805

Isotype: IgG1 kappa

Information d'application

Indications d'application: Applications include western blot analysis (0.2-5.0 µg/ml). HeLa nuclear extracts are recommended as a positive control.

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-MED23 (ABIN967286)

Blobel: "CREB-binding protein and p300: molecular integrators of hematopoietic transcription." dans: Blood, Vol. 95, Issue 3, pp. 745-55, (2000) (PubMed).

Boyer, Martin, Lees, Ricciardi, Berk: "Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein." dans: Nature, Vol. 399, Issue 6733, pp. 276-9, (1999) (PubMed).

Singh, Han: "sur-2, a novel gene, functions late in the let-60 ras-mediated signaling pathway during Caenorhabditis elegans vulval induction." dans: Genes & development, Vol. 9, Issue 18, pp. 2251-65, (1995) (PubMed).

Détails sur MED23

Antigène: MED23 (Mediator Complex Subunit 23 (MED23))

Autre désignation: Sur-2 (MED23 Produits)

Synonymes: anticorps CG3695, anticorps Dmel\\CG3695, anticorps Gal11/SUR2, anticorps Med23, anticorps Trap150, anticorps Trap150beta, anticorps dSUR2, anticorps dTRAP150beta, anticorps CRSP3, anticorps MED23, anticorps ARC130, anticorps CRSP130, anticorps CRSP133, anticorps DRIP130, anticorps MRT18, anticorps SUR-2, anticorps SUR2, anticorps 130kDa, anticorps 3000002A17Rik, anticorps Crsp3, anticorps ESTM7, anticorps Sur2, anticorps X83317, anticorps mKIAA1216, anticorps crsp3, anticorps si:ch211-272f15.2, anticorps zgc:91979, anticorps Mediator complex subunit 23, anticorps mediator complex subunit 23, anticorps mediator of RNA polymerase II transcription subunit 23, anticorps mediator complex subunit 23 L homeolog, anticorps Mediator of RNA polymerase II transcription subunit 23, anticorps MED23, anticorps med23, anticorps LOC412332, anticorps Med23, anticorps med23.L, anticorps sur-2

Sujet: SUR-2 (suppressor of ras) is a gene that was originally discovered in the vulval signaling pathway of the nematode Caenorhabditis elegans. Genetic studies suggest that the product of the SUR-2 gene encodes a protein Sur-2, that acts downstream of the Raf/Map kinase pathway. The human homolog of SUR-2 (hSUR-2) was shown to interact with the E1A zinc finger domain from adenovirus. The adenovirus E1A protein can bind to a number of cellular proteins to alter cell cycle progression. E1A exerts this change in the cell cycle by interacting with the transcriptional machinery of the cell. In vitro studies indicate that Sur-2 interacts with the E1A zinc finger protein and the Srb/Mediator transcriptional multiprotein complex. These studies suggest that Sur-2 may play a role as a bridge between E1A and the Srb/Mediator multiprotein complex. However, the roles of Sur-2 in regulating the transcriptional activity of the Srb/Mediator complex and E1A remain to be fully elucidated. Sur-2 has been reported to migrate in SDS/PAGE at 150 kDa2, but our studies indicate that it migrates at approximately 140 kDa. The antibody recognizes human Sur-2. A synthetic peptide corresponding to the N-terminus region of human Sur-2 (METQLQSIFEEVVKTEVIEC) was used as immunogen.

Poids moléculaire: 140 kDa

Pathways: Regulation of Lipid Metabolism by PPARalpha