MED23 anticorps (AA 897-916)

N° du produit ABIN6655801

Détail du produit anti-MED23 anticorps

Antigène: MED23 (Mediator Complex Subunit 23 (MED23))

Épitope: AA 897-916

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MED23 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée: Humain, Souris

Purification: This antiserum was processed by delipidation and defibrination and is directed against mouse DRIP130.  This reagent can be used to determine the presence of DRIP130 various immunological assays. Cross-reactivity occurs with human DRIP-130.  Reactivity with DRIP-130 isolated from other species is unknown.

Immunogène:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to mouse DRIP-130 residues  897-916 (based on human DRIP-130 residue numbering)  crosslinked to KLH.

Immunogen Type: Peptide

Information d'application

Indications d'application:

Application Note: Anti-DRIP-130 detects a 130 kDa band by immunoblot analysis using a dilution of 1:1,000. For immunoprecipitation a dilution of 1:100 or 1:200 is suggested, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. Optimal titers for applications should be determined by the researcher. Rockland Immunochemical's anti-DRIP130 has been used to detect DRIP130 using nuclear extracts from mouse thymus, spleen, and brain. Unfortunately, we have not tested reactivity on any cell line extracts. The following standard procedure is recommended: separate 10 micrograms of thymus nuclear extract by 7.5 % SDS-PAGE (0.75 to 1 mm thick). Transfer to nitrocellulose without SDS in the transfer buffer (pre-equilibrate the gel in transfer buffer for 30 min prior to transfer). Blocking is performed with 5 % non-fat dry milk in TTBS for 1 hr at room temperature. Incubate anti-DRIP130 antibody at a 1:1,000 for 1 hr at room temperature. Dilute Donkey anti-Rabbit IgG-HRP 1:5,000 and react 1 hr at room temperature. A predominant band at ~130 KDa is detected under these conditions. Control or normal rabbit serum fails to recognize the 130 kDa protein.

Immunoprecipitation Dilution: 1:100 - 1:200

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:1,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.1 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-MED23 (ABIN6655801)

Kasper, Fukuyama, Brindle: "T-cells null for the MED23 subunit of mediator express decreased levels of KLF2 and inefficiently populate the peripheral lymphoid organs." dans: PLoS ONE, Vol. 9, Issue 7, pp. e102076, (2015) (PubMed).

Détails sur MED23

Antigène: MED23 (Mediator Complex Subunit 23 (MED23))

Autre désignation: DRIP130 (MED23 Produits)

Synonymes: anticorps CG3695, anticorps Dmel\\CG3695, anticorps Gal11/SUR2, anticorps Med23, anticorps Trap150, anticorps Trap150beta, anticorps dSUR2, anticorps dTRAP150beta, anticorps CRSP3, anticorps MED23, anticorps ARC130, anticorps CRSP130, anticorps CRSP133, anticorps DRIP130, anticorps MRT18, anticorps SUR-2, anticorps SUR2, anticorps 130kDa, anticorps 3000002A17Rik, anticorps Crsp3, anticorps ESTM7, anticorps Sur2, anticorps X83317, anticorps mKIAA1216, anticorps crsp3, anticorps si:ch211-272f15.2, anticorps zgc:91979, anticorps Mediator complex subunit 23, anticorps mediator complex subunit 23, anticorps mediator of RNA polymerase II transcription subunit 23, anticorps mediator complex subunit 23 L homeolog, anticorps Mediator of RNA polymerase II transcription subunit 23, anticorps MED23, anticorps med23, anticorps LOC412332, anticorps Med23, anticorps med23.L, anticorps sur-2

Sujet:

Synonyms: rabbit anti-DRIP130antibody, Mediator of RNA polymerase II transcription subunit 23, Mediator complex subunit 23, Cofactor required for Sp1, transcriptional activation subunit 3, CRSP complex subunit 3, CRSP3, Transcriptional coactivator CRSP130, ARC130, DRIP130, KIAA1216, SUR2

Background: This antiserum recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130), and was tested for use in immunoprecipitation and immunoblotting.  DRIP-130 has been described elsewhere as Sur2, CRSP130, ARC130, NAT140, and TRAP150b.    DRIP-130 is component of a multi-protein complex termed DRIP that interacts with the vitamin D receptor (VDR), together, the DRIP proteins constitute a new cofactor complex.  DRIP binds to several nuclear receptors and mediates ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of two other cofactor complexes called ARC and TRAP, which are recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates.  Several DRIP/ARC/TRAP subunits are also components of other potentially related cofactors, such as CRSP, NAT, SMCC and the mouse Mediator, indicating that unique classes of activators may share common sets or subsets of cofactors. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.

Gene Name: MED23, DRIP130

ID gène: 9439, 28558074

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha