Desmin anticorps

N° du produit ABIN967294

Cet anticorps Souris Monoclonal détecte spécifiquement Desmin dans WB et IF. Il présente une réactivité envers Humain, Rat, Souris et Poulet et a été mentionné dans 4+ publications.

Aperçu rapide pour Desmin anticorps (ABIN967294)

Antigène: Desmin (DES)

Reactivité: Humain, Rat, Souris, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: RD301

Détail du produit anti-Desmin anticorps

Marque: BD Pharmingen™

Réactivité croisée: Souris, Rat (Rattus), Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified desmin from chicken gizzard

Isotype: IgG2b

Information d'application

Indications d'application: Applications include western blot analysis and immunofluorescence microscopy on frozen sections although IF is not tested. Mouse muscle lysate is recommended as a positive control.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Desmin (ABIN967294)

Schaart, Viebahn, Langmann, Ramaekers: "Desmin and titin expression in early postimplantation mouse embryos." dans: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, (1990) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." dans: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, (1987) (PubMed).

Détails sur Desmin

Antigène: Desmin (DES)

Autre désignation: Desmin

Sujet: Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Desmin belongs to the type III category of IF proteins which are predominantly expressed in muscle cells including cardiac, skeletal and smooth muscle. Furthermore, the expression of desmin is regulated in a stage and tissue-specific manner, since it is induced during terminal differentiation of skeletal muscle cells. In skeletal cardiac muscle cells, desmin is localized in the Z-disk region and at the intercalated disk and acts to stabilize sarcomeres in stimulated muscle. Desmin migrates in SDS/PAGE as a 53 kDa protein.

Poids moléculaire: 53 kDa