Syndecan 1 anticorps

N° du produit ABIN967377

Détail du produit anti-Syndecan 1 anticorps

Antigène: Syndecan 1 (SDC1)

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Syndecan 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: NAMRU mouse mammary gland epithelial cell line NMuMG

Clone: 281

Isotype: IgG2a kappa

Information d'application

Indications d'application: CD138 is expressed at low density on cells in the normal bone marrow, therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a bright third-step reagent, such as Streptavidin-PE. Other reported applications include western blot analysis using a cationic blotting membrane and a specific method, ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Détails sur Syndecan 1

Antigène: Syndecan 1 (SDC1)

Autre désignation: CD138 (SDC1 Produits)

Synonymes: anticorps CG10497, anticorps CT27296, anticorps Dmel\\CG10497, anticorps Dsyn, anticorps Syd, anticorps Syn, anticorps dSdc, anticorps l(2)10608, anticorps sdc, anticorps syd, anticorps syn, anticorps Syn1, anticorps cd138, anticorps synd1, anticorps xSyn1, anticorps syndecan1, anticorps Syndecan-1, anticorps XSyndecan-1, anticorps SDC1, anticorps CD138, anticorps SDC, anticorps SYND1, anticorps syndecan, anticorps AA408134, anticorps AA409076, anticorps Sstn, anticorps Synd, anticorps Synd1, anticorps syn-1, anticorps HSPG, anticorps SYNDECA, anticorps Syndecan, anticorps Syndecan, anticorps syndecan 1, anticorps Sdc, anticorps sdc1, anticorps SDC1, anticorps Sdc1

Sujet: The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Syndecan-1

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism