CD8 alpha anticorps (Biotin)

N° du produit ABIN967472

Il existe 14+ publications pour ce produit. L’anticorps anti-CD8 alpha Monoclonal Souris est utilisé pour la détection de CD8 alpha dans des échantillons de Rat. Il a été validé pour FACS et WB.

Aperçu rapide pour CD8 alpha anticorps (Biotin) (ABIN967472)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB)

Clone: OX-8

Détail du produit anti-CD8 alpha anticorps (Biotin)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: High-molecular-weight rat thymocyte glycoproteins

Isotype: IgG1 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD8 alpha (Biotin) (ABIN967472)

Mitnacht, Bischof, Torres-Nagel, Hünig: "Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 700-7, (1998) (PubMed).

Scriba, Grau, Steiniger: "Phenotype of rat monocytes during acute kidney allograft rejection: increased expression of NKR-P1 and reduction of CD43." dans: Scandinavian journal of immunology, Vol. 47, Issue 4, pp. 332-42, (1998) (PubMed).

Hirji, Lin, Befus: "A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 4, pp. 1833-40, (1997) (PubMed).

Wallgren, Karlsson-Parra, Korsgren: "The main infiltrating cell in xenograft rejection is a CD4+ macrophage and not a T lymphocyte." dans: Transplantation, Vol. 60, Issue 6, pp. 594-601, (1995) (PubMed).

Kühnlein, Park, Herrmann, Elbe, Hünig et al.: "Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T ..." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 979-86, (1994) (PubMed).

Classon, Brown, Garnett, Somoza, Barclay, Willis, Williams: "The hinge region of the CD8 alpha chain: structure, antigenicity, and utility in expression of immunoglobulin superfamily domains." dans: International immunology, Vol. 4, Issue 2, pp. 215-25, (1992) (PubMed).

Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." dans: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." dans: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, (1992) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." dans: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Johnson, Gagnon, Barclay, Williams: "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." dans: The EMBO journal, Vol. 4, Issue 10, pp. 2539-45, (1985) (PubMed).

Thomas, Green: "Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens." dans: European journal of immunology, Vol. 13, Issue 10, pp. 855-8, (1984) (PubMed).

Mason, Arthur, Dallman, Green, Spickett, Thomas: "Functions of rat T-lymphocyte subsets isolated by means of monoclonal antibodies." dans: Immunological reviews, Vol. 74, pp. 57-82, (1983) (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." dans: Immunology, Vol. 42, Issue 4, pp. 593-600, (1981) (PubMed).

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." dans: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, (1980) (PubMed).

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8a

Sujet: The OX-8 antibody reacts with the hinge-like membrane-proximal domain of the 32 kDa alpha chain of the CD8 differentiation antigen. A truncated CD8 alpha' isoform has not been detected in the rat. The CD8 alpha and beta chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). Intestinal intrapithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b. It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes. OX-8 antibody does not react with resting CD4+ T helper cells. CD8 is an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase Ick. Macrophages have also been reported to express CD8 alpha and beta chains, which are involved in signal transduction. Soluble OX-8 mAb partially blocks in vitro MLR and CTL activity. This antibody is routinely tested by flow cytometric analysis.

Pathways: TCR Signaling