PMS2 anticorps

N° du produit ABIN967525

Détail du produit anti-PMS2 anticorps

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PMS2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Marque: BD Pharmingen™

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Recombinant Human PMS2

Clone: A16-4

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4° C.

Références pour anticorps anti-PMS2 (ABIN967525)

Marsischky, Filosi, Kane, Kolodner: "Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair." dans: Genes & development, Vol. 10, Issue 4, pp. 407-20, (1996) (PubMed).

Cleaver: "It was a very good year for DNA repair." dans: Cell, Vol. 76, Issue 1, pp. 1-4, (1994) (PubMed).

Prolla, Christie, Liskay: "Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene." dans: Molecular and cellular biology, Vol. 14, Issue 1, pp. 407-15, (1994) (PubMed).

Prolla, Pang, Alani, Kolodner, Liskay: "MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast." dans: Science (New York, N.Y.), Vol. 265, Issue 5175, pp. 1091-3, (1994) (PubMed).

Su, Modrich: "Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5057-61, (1986) (PubMed).

Détails sur PMS2

Antigène: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Autre désignation: PMS2 (PMS2 Produits)

Synonymes: anticorps HNPCC4, anticorps PMS2CL, anticorps PMSL2, anticorps AW555130, anticorps Pmsl2, anticorps PMS1 homolog 2, mismatch repair system component, anticorps PMS1 homolog2, mismatch repair system component, anticorps mismatch repair endonuclease PMS2, anticorps PMS2, anticorps Pms2, anticorps LOC463257, anticorps LOC107984056

Sujet: The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time. In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins. The MutS protein initially recognizes and binds to mismatched DNA. Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism. When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability. The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals. In humans the proteins are called MutS homolog2 (MSH2), MutL homolog (MLH1), and PMS2 which is also a homolog of MutL. After MSH2 and a partner bind to a mismatched DNA duplex, the complex is joined by a heterodimer of MLH1 and PMS2 which together help facilitate the later steps in mismatch repair. Two other members of this family, MSH3 and MSH6, can also join the MSH2-containing complex to help facilitate repair. The reduced molecular weight of PMS2 is ~100 kDa. mAb A16-4 recognizes human and mouse PMS2. Recombinant human PMS2 (C-terminal half) was used as immunogen.

Poids moléculaire: 100 kDa

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response