Topoisomerase I anticorps

N° du produit ABIN967555

Cet anticorps Souris Monoclonal détecte spécifiquement Topoisomerase I dans WB. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour Topoisomerase I anticorps (ABIN967555)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: Western Blotting (WB)

Clone: C-21

Détail du produit anti-Topoisomerase I anticorps

Marque: BD Pharmingen™

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgM

Information d'application

Indications d'application: The C-21 antibody is recommended for western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL-1555) or K562 human leukemia cells (ATCC CCL-243) may be used as a positive control for this application.

Commentaires:

Related Products: ABIN967389, ABIN968533

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-Topoisomerase I (ABIN967555)

Tazi, Rossi, Labourier, Gallouzi, Brunel, Antoine: "DNA topoisomerase I: customs officer at the border between DNA and RNA worlds?" dans: Journal of molecular medicine (Berlin, Germany), Vol. 75, Issue 11-12, pp. 786-800, (1998) (PubMed).

Sugimoto, Tsukahara, Oh-hara, Isoe, Tsuruo: "Decreased expression of DNA topoisomerase I in camptothecin-resistant tumor cell lines as determined by a monoclonal antibody." dans: Cancer research, Vol. 50, Issue 21, pp. 6925-30, (1990) (PubMed).

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: DNA Topoisomerase I

Sujet: DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication. The correction of DNA damage, so called proofreading functions of the cell, is achieved by numerous excision-repair enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is routinely tested by western blot analysis of A-431 cell lysates.

Poids moléculaire: 100 kDa

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance