CTNNA1 anticorps (AA 729-906)

N° du produit ABIN967813

Cet anticorps Souris Monoclonal détecte spécifiquement CTNNA1 dans WB, IF et IP. Il présente une réactivité envers Humain, Souris, Rat, Chien et Poulet et a été mentionné dans 5+ publications.

Aperçu rapide pour CTNNA1 anticorps (AA 729-906) (ABIN967813)

Antigène: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CTNNA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 5-a

Détail du produit anti-CTNNA1 anticorps

Épitope: AA 729-906

Réactivité croisée: Humain, Poulet, Chien, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse alpha-Catenin aa. 729-906

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CTNNA1 (ABIN967813)

Baki, Marambaud, Efthimiopoulos, Georgakopoulos, Wen, Cui, Shioi, Koo, Ozawa, Friedrich, Robakis: "Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin/p120 association, and regulates stability and function of the cadherin/catenin adhesion complex." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 5, pp. 2381-6, (2001) (PubMed).

Giannini, Vivanco, Kypta: "alpha-catenin inhibits beta-catenin signaling by preventing formation of a beta-catenin*T-cell factor*DNA complex." dans: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 21883-8, (2000) (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." dans: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, (1999) (PubMed).

Hirano, Kimoto, Shimoyama, Hirohashi, Takeichi: "Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization." dans: Cell, Vol. 70, Issue 2, pp. 293-301, (1992) (PubMed).

Herrenknecht, Ozawa, Eckerskorn, Lottspeich, Lenter, Kemler: "The uvomorulin-anchorage protein alpha catenin is a vinculin homologue." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9156-60, (1991) (PubMed).

Détails sur CTNNA1

Antigène: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Autre désignation: alpha-Catenin

Sujet: The catenins (alpha-, beta-, and gamma-) are cytoplasmic proteins that bind to the highly conserved cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). These junctions are critical for cell-cell adhesion, signal transmission between neighboring cells, and for the anchoring of the actin cytoskeleton. alpha-Catenin (CAP102) shows homology to vinculin, while beta-Catenin is similar to plakoglobin or the Drosophila armadillo gene product. alpha-Catenin was identified as an E-Cadherin-associated protein, however, it also appears to interact with other cadherin family members. There are at least two subtypes of alpha-Catenin: alphaE-Catenin and alphaN-Catenin. The predominant form is known as alphaE-Catenin. It is ubiquitously expressed, but at low levels in the nervous system. The expression of alphaN-Catenin is more restricted and this form predominates in the brain. Increased tyrosine phosphorylation of adherens junction proteins can disrupt catenin-cadherin complexes, leading to changes in cell adhesion properties. It has been noted that down-regulation of this group of proteins often precedes metastasis. In fact, data suggests a correlation between deletions within the alpha-Catenin gene and the development of prostate cancer. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 102 kDa

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location