Cyclin B1 anticorps (AA 14-168)

N° du produit ABIN967834

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin B1 dans WB, IF, IP et IHC (f). Il présente une réactivité envers Humain et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour Cyclin B1 anticorps (AA 14-168) (ABIN967834)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 18-Cyclin B

Détail du produit anti-Cyclin B1 anticorps

Épitope: AA 14-168

Réactivité croisée: Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Cyclin B aa. 14-168

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Cyclin B1 (ABIN967834)

Miyoshi, Rosner, Nozawa, Byrd, Morgan, Landesman-Bollag, Xu, Seldin, Schmidt, Taketo, Robinson, Cardiff, Hennighausen: "Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors." dans: Oncogene, Vol. 21, Issue 36, pp. 5548-56, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Topper, Campbell, Tugendreich, Daum, Burke, Hieter, Gorbsky: "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis." dans: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 282-92, (2002) (PubMed).

Takasaki, Kogure, Takeuchi, Kaneda, Yano, Hirokawa, Hirose, Shirai, Hashimoto: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, (2001) (PubMed).

Mailand, Falck, Lukas, Syljuâsen, Welcker, Bartek, Lukas: "Rapid destruction of human Cdc25A in response to DNA damage." dans: Science (New York, N.Y.), Vol. 288, Issue 5470, pp. 1425-9, (2000) (PubMed).

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: Cyclin B

Sujet: The B-type cyclins serve as subunits of the major mitotic protein kinase in many different organisms. In mammals, Cyclin B is a subunit for the cdc2 (Cdk1) protein kinase and is synthesized during late S and G2 phases. Cyclin B associates with inactive cdc2 and facilitates phosphorylation of cdc2 at Thr-14 and Tyr-15. This phosphorylation maintains the inactive state until the end of G2. The inactive Cyclin B-cdc2 complex continues to accumulate in the cytoplasm until the CDC25 phosphatase dephosphorylates Thr-14 and Tyr-15, rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B becomes degraded. This degradation process appears to be ubiquitin-dependent and is necessary for the cell to exit mitosis. Also, the conserved domain in B-type cyclins, known as the P box, is required for CDC25 activation. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 62 kDa

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase