p130 anticorps (AA 26-367)

N° du produit ABIN967864

Cet anticorps Souris Monoclonal détecte spécifiquement p130 dans WB, IF, IHC et IP. Il présente une réactivité envers Humain, Rat, Souris, Blow Fly et Poulet et a été mentionné dans 5+ publications.

Aperçu rapide pour p130 anticorps (AA 26-367) (ABIN967864)

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivité: Humain, Rat, Souris, Blow Fly, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p130 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 10-Rb2

Détail du produit anti-p130 anticorps

Épitope: AA 26-367

Réactivité croisée: Souris, Rat (Rattus), Poulet, Mouche des fruits (Drosophila melanogaster)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Rb2 aa. 26-367

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-p130 (ABIN967864)

Fusaro, Wang, Chellappan: "Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosis." dans: Oncogene, Vol. 21, Issue 29, pp. 4539-48, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Laplantine, Rossi, Sahni, Basilico, Cobrinik: "FGF signaling targets the pRb-related p107 and p130 proteins to induce chondrocyte growth arrest." dans: The Journal of cell biology, Vol. 158, Issue 4, pp. 741-50, (2002) (PubMed).

Mayol, Graña, Baldi, Sang, Hu, Giordano: "Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain." dans: Oncogene, Vol. 8, Issue 9, pp. 2561-6, (1993) (PubMed).

Yeung, Bell, Testa, Mayol, Baldi, Graña, Klinga-Levan, Knudson, Giordano: "The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19." dans: Oncogene, Vol. 8, Issue 12, pp. 3465-8, (1993) (PubMed).

Détails sur p130

Antigène: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Autre désignation: Rb2

Sujet: The pRb2 protein shows a high degree of identity with pRb and the related p107 protein. Both pRb and p107 bind to the adenovirus E1A, SV40 large T antigen, and papillomavirus E7 viral proteins. This binding initiates the release of transcription factors which are required for the expression of cell cycle-regulated genes. pRb, pRb2, and p107 interact with a conserved motif in these three viral proteins. In the E1A protein, this area is known as transforming domain 2, which is required for growth activation. The E1A-binding domain in pRb and p107 is a conserved motif known as the pocket region which consists of conserved A and B regions separated by non-conserved spacers of different sizes in pRb and p107. It is the pocket regions of pRb and p107 that associate with the E2F transcription factor. pRb2 also contains the conserved pocket region suggesting that it has functional similarities to pRb and p107. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 130 kDa

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases