DAP Kinase 1 anticorps (AA 694-947)

N° du produit ABIN967885

Cet anticorps Souris Monoclonal détecte spécifiquement DAP Kinase 1 dans WB, IHC, IF et IP. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour DAP Kinase 1 anticorps (AA 694-947) (ABIN967885)

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DAP Kinase 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 17-DAP Kinase

Détail du produit anti-DAP Kinase 1 anticorps

Épitope: AA 694-947

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human DAP Kinase aa. 694-947

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-DAP Kinase 1 (ABIN967885)

Cohen, Feinstein, Kimchi: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." dans: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, (1997) (PubMed).

Deiss, Feinstein, Berissi, Cohen, Kimchi: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." dans: Genes & development, Vol. 9, Issue 1, pp. 15-30, (1995) (PubMed).

Détails sur DAP Kinase 1

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Autre désignation: DAP Kinase

Sujet: Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 160 kDa

Pathways: Signalisation MAPK, Interferon-gamma Pathway