NOS1 anticorps (AA 1095-1289)

N° du produit ABIN967894

Cet anticorps Souris Monoclonal détecte spécifiquement NOS1 dans WB, IHC, IP et IF. Il présente une réactivité envers Humain, Rat, Souris et Chien et a été mentionné dans 5+ publications.

Aperçu rapide pour NOS1 anticorps (AA 1095-1289) (ABIN967894)

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NOS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 16-nNOS-NOS Type I

Détail du produit anti-NOS1 anticorps

Épitope: AA 1095-1289

Réactivité croisée: Rat (Rattus), Souris, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human nNOS aa. 1095-1289

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NOS1 (ABIN967894)

Noorian, Taylor, Annerino, Greene: "Neurochemical phenotypes of myenteric neurons in the rhesus monkey." dans: The Journal of comparative neurology, Vol. 519, Issue 17, pp. 3387-401, (2012) (PubMed).

Schuh, Uldrijan, Telkamp, Rothlein, Neyses: "The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I." dans: The Journal of cell biology, Vol. 155, Issue 2, pp. 201-5, (2001) (PubMed).

Sasaki, Gonzalez-Zulueta, Huang, Herring, Ahn, Ginty, Dawson, Dawson: "Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8617-22, (2000) (PubMed).

Yu, Shao, Qian, George, Rockey: "Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension." dans: The Journal of clinical investigation, Vol. 105, Issue 6, pp. 741-8, (2000) (PubMed).

Nathan: "Nitric oxide as a secretory product of mammalian cells." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, (1992) (PubMed).

Détails sur NOS1

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Autre désignation: nNOS/NOS Type I

Sujet: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This antibody is routinely tested by western blot analysis.
Synonyms: NOS Type I, Neuronal Nitric Oxide Synthase

Poids moléculaire: 155 kDa

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction