NCF1 anticorps (AA 18-197)

N° du produit ABIN967918

Cet anticorps Souris Monoclonal détecte spécifiquement NCF1 dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 5+ publications.

Aperçu rapide pour NCF1 anticorps (AA 18-197) (ABIN967918)

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NCF1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1-p47Phox

Détail du produit anti-NCF1 anticorps

Épitope: AA 18-197

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human p47[phox] aa. 18-197

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-NCF1 (ABIN967918)

Shiose, Sumimoto: "Arachidonic acid and phosphorylation synergistically induce a conformational change of p47phox to activate the phagocyte NADPH oxidase." dans: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13793-801, (2000) (PubMed).

Ago, Nunoi, Ito, Sumimoto: "Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating" dans: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33644-53, (1999) (PubMed).

Hata, Ito, Takeshige, Sumimoto: "Anionic amphiphile-independent activation of the phagocyte NADPH oxidase in a cell-free system by p47phox and p67phox, both in C terminally truncated forms. Implication for regulatory Src homology 3 domain-mediated interactions." dans: The Journal of biological chemistry, Vol. 273, Issue 7, pp. 4232-6, (1998) (PubMed).

Chanock, el Benna, Smith, Babior: "The respiratory burst oxidase." dans: The Journal of biological chemistry, Vol. 269, Issue 40, pp. 24519-22, (1994) (PubMed).

Jackson, Malech, Kozak, Lomax, Gallin, Holland: "Cloning and functional expression of the mouse homologue of p47phox." dans: Immunogenetics, Vol. 39, Issue 4, pp. 272-5, (1994) (PubMed).

Détails sur NCF1

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Autre désignation: p47 phox

Sujet: The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. The enzyme is dormant in resting neutrophils. The active site of this enzyme is located in an integral membrane cytochrome, b558, which consists of the two subunits gp91[phox] and p21[phox]. Superoxide production depends on the formation of a complex that includes two cytosolic proteins, p67[phox] and p47[phox]. The GTP-binding protein Rac is also an essential component for oxidase activity. p47[phox] is a highly basic protein that contains two SH3 domains. The C-terminal quarter of the molecule contains many potential phosphorylation sites, consisting of serines and basic residues. Expression of p47[phox] is restricted to cells of phagocytic or lymphocytic lineage. IFN-gamma is a potent inducer of both p47[phox] mRNA and protein. p47[phox] is an early reactant in oxidase assembly and this assembly can be inhibited by a C-terminal peptide of the large subunit of cytochrome b558. It is thought that p47[phox] binds directly to the cytochrome, while p67[phox] associates with the cytochrome by binding p47[phox]. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 47 kDa

Pathways: Signalisation PI3K-Akt