NOS2 anticorps (AA 961-1144)

N° du produit ABIN967964

L’anticorps Souris Monoclonal anti-NOS2 a été validé pour WB, IHC, IP et IF. Il convient pour détecter NOS2 dans des échantillons de Souris. Il y a 5+ publications disponibles.

Aperçu rapide pour NOS2 anticorps (AA 961-1144) (ABIN967964)

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NOS2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 54-iNOS

Détail du produit anti-NOS2 anticorps

Épitope: AA 961-1144

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse iNOS aa. 961-1144

Isotype: IgG2a kappa

Information d'application

Commentaires:

Related Products: ABIN968550, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-NOS2 (ABIN967964)

Zadeh, Kolb, Geromin, DAnna, Boulmerka, Marconi, Dugas, Marsac, DAlessio: "Regulation of ICAM-1/CD54 expression on human endothelial cells by hydrogen peroxide involves inducible NO synthase." dans: Journal of leukocyte biology, Vol. 67, Issue 3, pp. 327-34, (2000) (PubMed).

Resta, ODonaughy, Earley, Chicoine, Walker: "Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats." dans: The American journal of physiology, Vol. 276, Issue 1 Pt 1, pp. L122-30, (1999) (PubMed).

Zhao, Dugas, Mathiot, Delmer, Dugas, Sigaux, Kolb: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." dans: Blood, Vol. 92, Issue 3, pp. 1031-43, (1998) (PubMed).

Vodovotz, Bogdan, Paik, Xie, Nathan: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." dans: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, (1993) (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." dans: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, (1992) (PubMed).

Détails sur NOS2

Antigène: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Autre désignation: iNOS/NOS Type II

Sujet: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. This antibody has been reported to cross-react with nNOS and eNOS.
Synonyms: NOS Type II

Poids moléculaire: 130 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process