CDC27 anticorps (AA 145-343)

N° du produit ABIN967981

Cet anticorps Souris Monoclonal détecte spécifiquement CDC27 dans WB, IHC, IP et IF. Il présente une réactivité envers Humain, Rat, Souris et Chien et a été mentionné dans 4+ publications.

Aperçu rapide pour CDC27 anticorps (AA 145-343) (ABIN967981)

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDC27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 35-CDC27

Détail du produit anti-CDC27 anticorps

Épitope: AA 145-343

Réactivité croisée: Chien, Rat (Rattus), Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human CDC27 aa. 145-343

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CDC27 (ABIN967981)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Kramer, Scheuringer, Podtelejnikov, Mann, Peters: "Mitotic regulation of the APC activator proteins CDC20 and CDH1." dans: Molecular biology of the cell, Vol. 11, Issue 5, pp. 1555-69, (2000) (PubMed).

Tugendreich, Tomkiel, Earnshaw, Hieter: "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition." dans: Cell, Vol. 81, Issue 2, pp. 261-8, (1995) (PubMed).

Tugendreich, Boguski, Seldin, Hieter: "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10031-5, (1993) (PubMed).

Détails sur CDC27

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Autre désignation: Cdc27

Sujet: CDC16 and CDC27 were discovered in yeast as genes essential for the G2/M transition of the cell cycle. The human homologues of CDC16 and CDC27 were cloned and found to encode proteins of 72kDa and 97kDa, respectively. Biochemical and subcellular localization experiments have shown that CDC16 and CDC27 are novel components of the mitotic spindle centrosome in human cells. In HeLa cells, there are about 100,000 copies of each protein per cell. Although its precise role during mitosis remains to be determined, CDC16 appears to be required for cyclin degradation in yeast. Wild type CDC27 is required for the initiation of anaphase. Immunofluorescence studies have shown that both proteins are components of the centrosome in the absence of spindle fibers.

Poids moléculaire: 97 kDa