Sec8 anticorps (AA 31-201)

N° du produit ABIN968092

Cet anticorps Souris Monoclonal détecte spécifiquement Sec8 dans WB, IHC, IP et IF. Il présente une réactivité envers Humain, Souris, Rat, Chien et Poulet et a été mentionné dans 3+ publications.

Aperçu rapide pour Sec8 anticorps (AA 31-201) (ABIN968092)

Antigène: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Sec8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 14-Sec8

Détail du produit anti-Sec8 anticorps

Épitope: AA 31-201

Réactivité croisée: Chien, Souris, Poulet, Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Sec8 aa. 31-201

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Sec8 (ABIN968092)

Charron, Nakamura, Bacallao, Wandinger-Ness: "Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells." dans: The Journal of cell biology, Vol. 149, Issue 1, pp. 111-24, (2000) (PubMed).

Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." dans: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, (1999) (PubMed).

Ting, Hazuka, Hsu, Kirk, Bean, Scheller: "rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9613-7, (1995) (PubMed).

Détails sur Sec8

Antigène: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Autre désignation: Sec8

Sujet: Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 110 kDa

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis