UBE2I anticorps (AA 26-156)

N° du produit ABIN968155

Cet anticorps Souris Monoclonal détecte spécifiquement UBE2I dans WB, IHC, IF et IP. Il présente une réactivité envers Humain, Souris, Rat, Poulet et Chien et a été mentionné dans 4+ publications.

Aperçu rapide pour UBE2I anticorps (AA 26-156) (ABIN968155)

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Reactivité: Humain, Souris, Rat, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UBE2I est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 50-Ubc9

Détail du produit anti-UBE2I anticorps

Épitope: AA 26-156

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Ubc9 aa. 26-156

Isotype: IgG2a

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-UBE2I (ABIN968155)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Buschmann, Lerner, Lee, Ronai: "The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9." dans: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40389-95, (2001) (PubMed).

Kovalenko, Plug, Haaf, Gonda, Ashley, Ward, Radding, Golub: "Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 7, pp. 2958-63, (1996) (PubMed).

Wang, Qiu, Seufert, Taguchi, Testa, Whitmore, Callen, Welsh, Shenk, Deuel: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." dans: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, (1996) (PubMed).

Détails sur UBE2I

Antigène: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Autre désignation: Ubc9

Sujet: Progression of the mammalian cell cycle is primarily regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an e-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome (a cytoplasmic complex of proteases). Human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been reported, including Ubc9. Ubc9 is 158 amino acids with an apparent molecular weight of 18kDa. Although ubiquitously expressed, the highest levels of Ubc9 are found in testis and thymus. Ubc9 was localized to the synaptonemal complex in male mouse sex chromosomes. Furthermore, Ubc9 interacts with the recombination protein Rad51, thus suggesting an important role for Ubc9 during meiosis.

Poids moléculaire: 18 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway