CASK anticorps (AA 353-486)

N° du produit ABIN968174

L’anticorps Souris Monoclonal anti-CASK a été validé pour WB, IHC, IF et IP. Il convient pour détecter CASK dans des échantillons de Humain, Souris, Rat, Chien et Grenouille. Il y a 5+ publications disponibles.

Aperçu rapide pour CASK anticorps (AA 353-486) (ABIN968174)

Antigène: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Reactivité: Humain, Souris, Rat, Chien, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CASK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 7-CASK

Détail du produit anti-CASK anticorps

Épitope: AA 353-486

Réactivité croisée: Souris, Humain, Chien, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat CASK aa. 353-486

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CASK (ABIN968174)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." dans: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Biederer, Sudhof: "CASK and protein 4.1 support F-actin nucleation on neurexins." dans: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47869-76, (2001) (PubMed).

Borg, Lõpez-Figueroa, de Taddèo-Borg, Kroon, Turner, Watson, Margolis: "Molecular analysis of the X11-mLin-2/CASK complex in brain." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 4, pp. 1307-16, (1999) (PubMed).

Irie, Hata, Takeuchi, Ichtchenko, Toyoda, Hirao, Takai, Rosahl, Südhof: "Binding of neuroligins to PSD-95." dans: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1511-5, (1997) (PubMed).

Hata, Butz, Südhof: "CASK: a novel dlg/PSD95 homolog with an N-terminal calmodulin-dependent protein kinase domain identified by interaction with neurexins." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 8, pp. 2488-94, (1996) (PubMed).

Détails sur CASK

Antigène: CASK (Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))

Autre désignation: CASK

Sujet: CASK is a recently identified cytosolic protein kinase with homology to the Ca2+/CaM-dependent kinases and the synaptic associated proteins SAPs/PSDs. Like the SAPs, CASK contains a PDZ domain, an SH3 region, and a guanylate kinase domain. However, unlike the rest of the PDZ protein family, the amino terminus of CASK has significant homology with the Ca2+/Calmodulin-dependent kinases. Although widely expressed, CASK is highly enriched in the synaptic plasma membrane where it associates with neurexins, the neuronal cell surface proteins. Neurexins are a complex family of surface proteins that act as receptors for a number of venoms and toxins and regulate the clustering of several ion channels at the synapse. In addition, neurexins bind heterotypically to neuroligins, therefore adjoining different cell types. Neuroligins bind intracellularly to PSD95 and related proteins, whereas neurexins bind to CASK through their C-terminal region and at CASK's PDZ domain. The interaction of neurexins and CASK at the outside of the cell may modulate CASK's activity and trigger an intracellular signaling cascade.

Poids moléculaire: 120 kDa

Pathways: Synaptic Vesicle Exocytosis