FKBP1A anticorps (AA 1-108)

N° du produit ABIN968189

L’anticorps Souris Monoclonal anti-FKBP1A a été validé pour WB, IHC, IP et IF. Il convient pour détecter FKBP1A dans des échantillons de Humain, Souris, Chien et Poulet. Il y a 2+ publications disponibles.

Aperçu rapide pour FKBP1A anticorps (AA 1-108) (ABIN968189)

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Reactivité: Humain, Souris, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FKBP1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 8-FKBP12

Détail du produit anti-FKBP1A anticorps

Épitope: AA 1-108

Réactivité croisée: Souris, Chien, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human FKBP12 aa. 1-108

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968552, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-FKBP1A (ABIN968189)

Chen, Liu, Massague: "Mechanism of TGFbeta receptor inhibition by FKBP12." dans: The EMBO journal, Vol. 16, Issue 13, pp. 3866-76, (1997) (PubMed).

Wang, Donahoe, Zervos: "Specific interaction of type I receptors of the TGF-beta family with the immunophilin FKBP-12." dans: Science (New York, N.Y.), Vol. 265, Issue 5172, pp. 674-6, (1994) (PubMed).

Détails sur FKBP1A

Antigène: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Autre désignation: FKBP12

Sujet: Transforming growth factor-beta binds to the TGFbeta family of heteromeric serine/threonine transmembrane receptors (type I and type II). Following binding of TGFbeta, the type II receptor (TbetaR-II) phosphorylates the type I receptor (TbetaR-I) which, in turn, conveys the signal. Since TbetaR-I and TbetaR-II can interact without the stimulation of TGFbeta, leading to unwanted activation, a regulatory mechanism exists. In a yeast genetic screen, immunophilin FKBP12 was associated with the type I receptor. Studies including co-immunoprecipiation, deletion, and point mutations confirmed this interaction. FKBP12 inhibits TbetaR-II mediated phosphorylation of TbetaR-I, inhibiting activation. FKBP12 binds via its rapamycin/Leu-Pro binding pocket to the Leu-Pro sequence adjacent to the phosphorylation site of TbetaR-I. This interaction is blocked by the addition of macrolides, rapamycin, and FK506. Furthermore, mutations in the binding sites of FKBP12 and TbetaR-I abrogates the binding and results in activation of the receptor without the addition of TGFbeta. Thus, FKBP12 is a regulatory protein for TbetaR-I and TbetaR-II-mediated signaling.
Synonyms: FK506 Binding Protein 1A

Poids moléculaire: 14 kDa

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process