SRP54 anticorps (AA 262-476)

N° du produit ABIN968262

Cet anticorps anti-SRP54 est un anticorps Souris Monoclonal détectant SRP54 dans WB et IF. Adapté pour Humain, Souris, Rat, Chien et Grenouille. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour SRP54 anticorps (AA 262-476) (ABIN968262)

Antigène: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Reactivité: Humain, Souris, Rat, Chien, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SRP54 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 30-SRP54

Détail du produit anti-SRP54 anticorps

Épitope: AA 262-476

Réactivité croisée: Chien, Rat (Rattus), Souris, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human SRP54 aa. 262-476

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SRP54 (ABIN968262)

Rapiejko, Gilmore: "Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum." dans: Cell, Vol. 89, Issue 5, pp. 703-13, (1997) (PubMed).

Traianedes, Findlay, Martin, Gillespie: "Modulation of the signal recognition particle 54-kDa subunit (SRP54) in rat preosteoblasts by the extracellular matrix." dans: The Journal of biological chemistry, Vol. 270, Issue 36, pp. 20891-4, (1995) (PubMed).

Détails sur SRP54

Antigène: SRP54 (Signal Recognition Particle 54kDa (SRP54))

Autre désignation: SRP54

Sujet: Ribosomes that exist freely in the cytosol or those attached to the ER are intrinsically the same in their translational properties. ER-bound ribosomes are responsible for the production of secretory proteins and integral ER, Golgi, lysosomal, and plasma membrane spanning proteins. Such proteins contain signal sequences that direct their synthesis to the ER membrane. As the nascent polypeptide emerges from the ribosome, a signal recognition particle (SRP) binds to the signal sequence and serves to couple the ribosome to the protein-translocating machinery in the ER membrane. Although the SRP is a 325 kDa ribonucleoprotein, its 54 kDa subunit (SRP54) mediates interaction with, and targeting of, the nascent protein to the ER. Via its C-terminal M-domain, SRP54 associates with the nascent protein and inhibits its elongation. This complex binds to the SRP receptor on the ER, the ribosome is delivered to the translocation machinery, SRP is released, and elongation resumes. Targeting and insertion are tightly coupled to a GTPase cycle that involves SRP54 and SRP receptor. Although the mechanisms are unclear, release of SRP from the ER-bound complex requires GTP hydrolysis.

Poids moléculaire: 54 kDa

Pathways: SARS-CoV-2 Protein Interactome