TJP1 anticorps (AA 1048-1247)

N° du produit ABIN968283

Cet anticorps anti-TJP1 est un anticorps Souris Monoclonal détectant TJP1 dans WB et IF. Adapté pour Humain. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour TJP1 anticorps (AA 1048-1247) (ABIN968283)

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TJP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1-ZO

Détail du produit anti-TJP1 anticorps

Épitope: AA 1048-1247

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human ZO-1 aa. 1048-1247

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-TJP1 (ABIN968283)

Hirase, Kawashima, Wong, Ueyama, Rikitake, Tsukita, Yokoyama, Staddon: "Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms." dans: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10423-31, (2001) (PubMed).

Nix, Chishti, Anderson, Walther: "hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions." dans: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41192-200, (2001) (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." dans: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, (2000) (PubMed).

Willott, Balda, Fanning, Jameson, Van Itallie, Anderson: "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7834-8, (1993) (PubMed).

Détails sur TJP1

Antigène: TJP1 (Tight Junction Protein 1 (TJP1))

Autre désignation: ZO-1

Sujet: Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intracellular barriers between epithelial and endothelial cells. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1, and ZO-2. Analysis of ZO-1 and -2 resulted in their inclusion in the MAGUK protein family. This family also includes the discs large tumor suppressor protein (Dlg-A) of Drosophila, p55, an erythrocyte membrane protein, and PSD-95/SAP90, a synaptic membrane protein. All family members contain a region homologous to guanylate kinase (GuK), a src homology (SH3) domain, and multiple PDZ domains. Through these elements, MAGUK proteins function in signal transduction and, possibly, tumor suppression. ZO-1 is a peripheral membrane phosphoprotein that binds to other tight junction proteins such as occludin and AF-6. Via its SH3 domain, ZO-1 interacts with a serine protein kinase that phosphorylates a region immediately C-terminal of the SH3 domain. Taken together, these data indicate that ZO-1 is a critical element in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 220 kDa

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization