PP5 anticorps (AA 36-238)

N° du produit ABIN968321

L’anticorps Souris Monoclonal anti-PP5 a été validé pour WB et IF. Il convient pour détecter PP5 dans des échantillons de Humain, Rat, Souris et Chien. Il y a 5+ publications disponibles.

Aperçu rapide pour PP5 anticorps (AA 36-238) (ABIN968321)

Antigène: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PP5 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 3-PP5

Détail du produit anti-PP5 anticorps

Épitope: AA 36-238

Réactivité croisée: Souris, Humain, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat PP5 aa. 36-238

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PP5 (ABIN968321)

Das, Cohen, Barford: "The structure of the tetratricopeptide repeats of protein phosphatase 5: implications for TPR-mediated protein-protein interactions." dans: The EMBO journal, Vol. 17, Issue 5, pp. 1192-9, (1998) (PubMed).

Ollendorff, Donoghue: "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex." dans: The Journal of biological chemistry, Vol. 272, Issue 51, pp. 32011-8, (1998) (PubMed).

Zuo, Dean, Honkanen: "Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21(WAF1/Cip1) and mediate growth arrest." dans: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12250-8, (1998) (PubMed).

Becker, Kentrup, Klumpp, Schultz, Joost: "Molecular cloning of a protein serine/threonine phosphatase containing a putative regulatory tetratricopeptide repeat domain." dans: The Journal of biological chemistry, Vol. 269, Issue 36, pp. 22586-92, (1994) (PubMed).

Chen, McPartlin, Brown, Chen, Barker, Cohen: "A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus." dans: The EMBO journal, Vol. 13, Issue 18, pp. 4278-90, (1994) (PubMed).

Détails sur PP5

Antigène: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Autre désignation: PP5

Sujet: Phosphorylation and dephosphorylation on serine and threonine residues is critical for signal transduction and the regulation of numerous cellular functions. Phosphorylation levels are modulated by protein kinases and phosphatases. The Ser/Thr phosphatase PP5 (also known as PPT) is a model protein of the fourth subfamily of the PPP-family of phosphatases which includes PP1, PP2A, and PP2B. PP5 is primarily located in the nucleus, demonstrates widespread tissue distribution, and is highly conserved among species. It consists of a C-terminal catalytic domain and an N-terminal TPR (tetratricopeptide repeat) domain which is involved in protein-protein interactions. The TPRs of PP5 mediate protein phosphatase activity. TPRs mediate the interaction of PP5 with various proteins including the hsp90-glucocorticoid receptor complex and the kinase domain of ANP-guanylate cyclase receptor. PP5 is thought to be important in cell growth. For example, it functions upstream of p53 to regulate p21/WAF1/Cip1-mediated G1 growth arrest. Thus, PP5 is a nuclear protein phosphatase that is involved in signaling pathways of cell growth and arrest. This antibody is routinely tested by western blot analysis.
Synonyms: PPT

Poids moléculaire: 58 kDa