MAP4 anticorps (AA 583-702)

N° du produit ABIN968326

Cet anticorps Souris Monoclonal détecte spécifiquement MAP4 dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 3+ publications.

Aperçu rapide pour MAP4 anticorps (AA 583-702) (ABIN968326)

Antigène: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAP4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 18-MAP4

Détail du produit anti-MAP4 anticorps

Épitope: AA 583-702

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MAP4 aa. 583-702

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-MAP4 (ABIN968326)

Drewes, Ebneth, Preuss, Mandelkow, Mandelkow: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." dans: Cell, Vol. 89, Issue 2, pp. 297-308, (1997) (PubMed).

Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, (1997) (PubMed).

Chapin, Lue, Yu, Bulinski: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." dans: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, (1995) (PubMed).

Détails sur MAP4

Antigène: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Autre désignation: MAP4

Sujet: The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.

Poids moléculaire: 200-220 kDa

Pathways: Signalisation p53, Dynamique des Microtubules