VTI1A anticorps (AA 114-217)

N° du produit ABIN968427

Cet anticorps anti-VTI1A est un anticorps Souris Monoclonal détectant VTI1A dans WB et IF. Adapté pour Humain, Souris, Rat et Chien. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour VTI1A anticorps (AA 114-217) (ABIN968427)

Antigène: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VTI1A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 45-Vti1a

Détail du produit anti-VTI1A anticorps

Épitope: AA 114-217

Réactivité croisée: Rat (Rattus), Humain, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Vti1a aa.114-217

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-VTI1A (ABIN968427)

Chiu, Novikov, Mukherjee, Shields: "A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis." dans: The Journal of cell biology, Vol. 159, Issue 4, pp. 637-48, (2002) (PubMed).

Mallard, Tang, Galli, Tenza, Saint-Pol, Yue, Antony, Hong, Goud, Johannes: "Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform." dans: The Journal of cell biology, Vol. 156, Issue 4, pp. 653-64, (2002) (PubMed).

Shorter, Beard, Seemann, Dirac-Svejstrup, Warren: "Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115." dans: The Journal of cell biology, Vol. 157, Issue 1, pp. 45-62, (2002) (PubMed).

Advani, Bae, Bock, Chao, Doung, Prekeris, Yoo, Scheller: "Seven novel mammalian SNARE proteins localize to distinct membrane compartments." dans: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10317-24, (1998) (PubMed).

Xu, Wong, Tang, Subramaniam, Zhang, Hong: "A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway." dans: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21783-9, (1998) (PubMed).

Détails sur VTI1A

Antigène: VTI1A (Vesicle Transport through Interaction with t-SNAREs 1A (VTI1A))

Autre désignation: Vti1a

Sujet: Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.

Poids moléculaire: 29 kDa