MSH3 anticorps (AA 136-349)

N° du produit ABIN968513

L’anticorps Souris Monoclonal anti-MSH3 a été validé pour WB et IF. Il convient pour détecter MSH3 dans des échantillons de Humain. Il y a 4+ publications disponibles.

Aperçu rapide pour MSH3 anticorps (AA 136-349) (ABIN968513)

Antigène: MSH3 (MutS Homolog 3 (MSH3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 52-MSH3

Détail du produit anti-MSH3 anticorps

Épitope: AA 136-349

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MSH3 aa. 136-349

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968535

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-MSH3 (ABIN968513)

Wilson, Guerrette, Fishel: "Dissociation of mismatch recognition and ATPase activity by hMSH2-hMSH3." dans: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21659-64, (1999) (PubMed).

Umar, Risinger, Glaab, Tindall, Barrett, Kunkel: "Functional overlap in mismatch repair by human MSH3 and MSH6." dans: Genetics, Vol. 148, Issue 4, pp. 1637-46, (1998) (PubMed).

Watanabe, Ikejima, Suzuki, Shimada: "Genomic organization and expression of the human MSH3 gene." dans: Genomics, Vol. 31, Issue 3, pp. 311-8, (1997) (PubMed).

New, Liu, Crouse: "The yeast gene MSH3 defines a new class of eukaryotic MutS homologues." dans: Molecular & general genetics : MGG, Vol. 239, Issue 1-2, pp. 97-108, (1993) (PubMed).

Détails sur MSH3

Antigène: MSH3 (MutS Homolog 3 (MSH3))

Autre désignation: MSH3

Sujet: Bacterial mismatch DNA repair involves the MutL, MutH, and MutS proteins, which forms a complex that mediates excision repair. Mutations in or deficiencies of any of these proteins results in a mutator phenotype that is characterized by genetic instability. Human homologs of MutS include MSH2, MSH3, and MSH6. MSH2 forms heterodimers with MSH6 (hMutSalpha) or MSH3 (hMutSbeta) that specifically bind single-mispaired nucleotides and a subset of insertion-deletion mismatches. In addition, these heterodimers have intrinsic ATPase activity that is regulated by mismatch binding. ADP-bound heterodimers bind mismatched nucleotides, while ATP-bound heterodimers do not. The role of MSH3 in genetic stability in human cells in unclear. However, MSH3 and MSH6 share roles in the control of mutation rates. Both participate in repair of replication errors containing base-base mismatches or 1-4 extra bases. The MSH3 gene is located upstream of the dihydrofolate reductase (DHFR) gene and is expressed at low levels in a variety of human tissues. Thus, MSH3 is a component of an adenosine nucleotide-regulated molecular switch whose activity is essential for classical nucleotide mismatch repair.

Poids moléculaire: 127 kDa

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response