ube3a anticorps (AA 501-712)

N° du produit ABIN968525

L’anticorps Souris Monoclonal anti-ube3a a été validé pour WB et IF. Il convient pour détecter ube3a dans des échantillons de Humain et Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour ube3a anticorps (AA 501-712) (ABIN968525)

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ube3a est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 13-UBE3A

Détail du produit anti-ube3a anticorps

Épitope: AA 501-712

Réactivité croisée: Humain

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse UBE3A aa. 501-712

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968584, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ube3a (ABIN968525)

Nawaz, Lonard, Smith, Lev-Lehman, Tsai, Tsai, OMalley: "The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily." dans: Molecular and cellular biology, Vol. 19, Issue 2, pp. 1182-9, (1999) (PubMed).

Matsuura, Sutcliffe, Fang, Galjaard, Jiang, Benton, Rommens, Beaudet: "De novo truncating mutations in E6-AP ubiquitin-protein ligase gene (UBE3A) in Angelman syndrome." dans: Nature genetics, Vol. 15, Issue 1, pp. 74-7, (1997) (PubMed).

Détails sur ube3a

Antigène: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Autre désignation: UBE3A

Sujet: Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. UBE3A (human papilloma virus E6-associated protein, E6-AP) is an E3 ubiquitin protein ligase that is linked to the neurological disorder Angelman syndrome. It mediates the interaction of human papilloma virus types 16 and 18 E6 proteins with p53, which targets p53 to the ubiquitination-proteasome degradation pathway. The C-terminal region of UBE3A contains a hect domain, which is found in many E3 ubiquitin protein ligases, while the central region contains an E6 binding region. UBE3A is expressed in the cytoplasm and nucleus of many tissues. The interaction of UBE3A with the progesterone receptor coactivates transciptional activity of the receptor in the presence of bound hormone. Thus, UBE3A is an essential component of the ubiquitination degradation pathway and the nuclear hormone receptor signal transduction pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Ubiquitin Protein Ligase 3A

Poids moléculaire: 100 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway