Phospholipase C beta 4 anticorps (AA 752-961)

N° du produit ABIN968583

Cet anticorps anti-Phospholipase C beta 4 est un anticorps Souris Monoclonal détectant Phospholipase C beta 4 dans WB, BI et IF. Adapté pour Humain, Rat, Souris et Blow Fly. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour Phospholipase C beta 4 anticorps (AA 752-961) (ABIN968583)

Antigène: Phospholipase C beta 4 (PLCb4)

Reactivité: Humain, Rat, Souris, Blow Fly

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phospholipase C beta 4 est non-conjugé

Application: Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)

Clone: 56-Phospholipase Cbeta4

Détail du produit anti-Phospholipase C beta 4 anticorps

Épitope: AA 752-961

Réactivité croisée: Rat (Rattus), Souris, Mouche des fruits (Drosophila melanogaster)

Attributs du produit: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Phospholipase Cbeta4 aa. 752-961

Isotype: IgG1

Information d'application

Indications d'application: Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Phospholipase C beta 4 (ABIN968583)

Kano, Hashimoto, Watanabe, Kurihara, Offermanns, Jiang, Wu, Jun, Shin, Inoue, Simon, Wu: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, (1999) (PubMed).

Kim, Jun, Lee, Kang, Min, Kim, Ryu, Suh, Shin: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." dans: Nature, Vol. 389, Issue 6648, pp. 290-3, (1997) (PubMed).

Alvarez, Ghalayini, Xu, Hardcastle, Bhattacharya, Rao, Pettenati, Anderson, Baehr: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." dans: Genomics, Vol. 29, Issue 1, pp. 53-61, (1996) (PubMed).

Lee, Park, Lee, Kim, Rhee: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." dans: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, (1993) (PubMed).

Détails sur Phospholipase C beta 4

Antigène: Phospholipase C beta 4 (PLCb4)

Autre désignation: Phospholipase C beta 4

Sujet: Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
Synonyms: PLCbeta4

Poids moléculaire: 130 kDa

Pathways: Signalisation WNT, Thyroid Hormone Synthesis, G-protein mediated Events