Synaptophysin anticorps (AA 205-306)

N° du produit ABIN968694

Cet anticorps anti-Synaptophysin est un anticorps Souris Monoclonal détectant Synaptophysin dans WB, IF et BI. Adapté pour Humain, Rat et Souris. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour Synaptophysin anticorps (AA 205-306) (ABIN968694)

Antigène: Synaptophysin (SYP)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Synaptophysin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Clone: 2-Synaptophysin

Détail du produit anti-Synaptophysin anticorps

Épitope: AA 205-306

Réactivité croisée: Humain, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Synaptophysin aa. 205-306

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Synaptophysin (ABIN968694)

Daly, Sugimori, Moreira, Ziff, Llinás: "Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 6120-5, (2000) (PubMed).

Thiele, Hannah, Fahrenholz, Huttner: "Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles." dans: Nature cell biology, Vol. 2, Issue 1, pp. 42-9, (2000) (PubMed).

Leube, Kaiser, Seiter, Zimbelmann, Franke, Rehm, Knaus, Prior, Betz, Reinke: "Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA." dans: The EMBO journal, Vol. 6, Issue 11, pp. 3261-8, (1988) (PubMed).

Détails sur Synaptophysin

Antigène: Synaptophysin (SYP)

Autre désignation: Synaptophysin

Sujet: Synaptophysin is a major integral membrane glycoprotein that was isolated from the presynaptic vesicles of neurons. The structure of synaptophysin includes four transmembrane domains (TMD), an N-temrinal glycosylation site, nine repeats of basic tetrapeptide motifs (GYGP), multiple collagenase sensitive sites (XGP), and several collagen-type tripeptides (GXX). Synaptophysin mRNA is expressed in nervous tissues, as well as many neuroendocrine tumor cell lines. In PC12 cells and brain synaptic vesicles, synaptophysin binds cholesterol, and cholesterol depletion blocks the biogenesis of synaptic-like microvesicles. In addition, synaptophysin binds dynamin in a Ca2+-dependent manner, and injection of peptides that disrupt this interaction decreases neurotransmitter release during high-frequency stimulation of squid neurons. This decrease in neurotransmitter release results from inhibition of synaptophysin-dependent vesicle retrieval. Thus, synaptophysin may have important roles in the biogenesis and recycling of synaptic vesicles. Synaptophysin is an integral membrane glycoprotein with a calculated molecular weight of 33 kD, but has been reported to be observed at 38-42 kD due to glycosylation. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 38 kDa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity