ARHGAP4 anticorps (AA 843-955)

N° du produit ABIN968790

Détail du produit anti-ARHGAP4 anticorps

Antigène: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Épitope: AA 843-955

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ARHGAP4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat p115 aa. 843-955

Clone: 15-p115

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ARHGAP4 (ABIN968790)

Mary, Charrasse, Meriane, Comunale, Travo, Blangy, Gauthier-Rouvière: "Biogenesis of N-cadherin-dependent cell-cell contacts in living fibroblasts is a microtubule-dependent kinesin-driven mechanism." dans: Molecular biology of the cell, Vol. 13, Issue 1, pp. 285-301, (2002) (PubMed).

Barroso, Nelson, Sztul: "Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 2, pp. 527-31, (1995) (PubMed).

Waters, Clary, Rothman: "A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack." dans: The Journal of cell biology, Vol. 118, Issue 5, pp. 1015-26, (1992) (PubMed).

Détails sur ARHGAP4

Antigène: ARHGAP4 (rho GTPase Activating Protein 4 (ARHGAP4))

Autre désignation: p115 (ARHGAP4 Produits)

Synonymes: anticorps zgc:63672, anticorps ARHGAP4, anticorps C1, anticorps RGC1, anticorps RhoGAP4, anticorps SrGAP4, anticorps p115, anticorps A130009C12Rik, anticorps A530015A20Rik, anticorps Rgc1, anticorps c1, anticorps mKIAA0131, anticorps Rho GTPase activating protein 4a, anticorps Rho GTPase activating protein 4, anticorps arhgap4a, anticorps ARHGAP4, anticorps arhgap4, anticorps Arhgap4

Sujet: Maturation and post translational modification of proteins occurs after their biosynthesis at the endoplasmic reticulum and their transport through the Golgi apparatus. The process involves the transport of vesicles carrying the proteins through a vectorial process of vesicle budding and fusion from the cis-compartment to the medial-compartment and the trans-compartment of the Golgi apparatus. p115 is a 959 amino acid protein located at the Golgi apparatus that, with the NEM-sensitive fusion protein and the soluble NSF attachment protein (SNAP), is required for vesicle transport from the cis-compartment to the medial-compartment. p115 protein is related to the yeast Uso1p essential for the vesicular transport from the endoplasmic reticulum to the Golgi. Native p115 appears to be a homo-oligomer, with two globular heads and a tail that resemble the overall structure of myosin. p115 is extracted from the Golgi apparatus with high salt or high pH, indicative of a membrane associated protein. p115 interacts with the golgi matrix protein GM130 but this interaction is disrupted by the Golgi fragmentation during mitosis and the phosphorylation of GM130. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 115 kDa

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size