MAD2L2 anticorps (AA 81-180)

N° du produit ABIN968795

L’anticorps Souris Monoclonal anti-MAD2L2 a été validé pour WB et IF. Il convient pour détecter MAD2L2 dans des échantillons de Humain et Chien. Il y a 2+ publications disponibles.

Aperçu rapide pour MAD2L2 anticorps (AA 81-180) (ABIN968795)

Antigène: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAD2L2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 14-MAD2B-Rev7

Détail du produit anti-MAD2L2 anticorps

Épitope: AA 81-180

Réactivité croisée: Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human MAD2B aa. 81-180

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-MAD2L2 (ABIN968795)

Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." dans: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).

Murakumo, Roth, Ishii, Rasio, Numata, Croce, Fishel: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." dans: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, (2000) (PubMed).

Détails sur MAD2L2

Antigène: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Autre désignation: MAD2B/Rev7

Sujet: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase zeta subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression. This antibody is routinely tested by western blot analysis.
Synonyms: Mitotic Arrest Deficient-2B, Rev7

Poids moléculaire: 26 kDa