SEC31A anticorps (AA 522-719)

N° du produit ABIN968830

Cet anticorps Souris Monoclonal détecte spécifiquement SEC31A dans WB et IF. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour SEC31A anticorps (AA 522-719) (ABIN968830)

Antigène: SEC31A (SEC31 Homolog A (SEC31A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SEC31A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 32-Sec31A

Détail du produit anti-SEC31A anticorps

Épitope: AA 522-719

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Sec31A aa. 522-719

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SEC31A (ABIN968830)

Tang, Ong, Huang, Wei, Wong, Qi, Horstmann, Hong: "A membrane protein enriched in endoplasmic reticulum exit sites interacts with COPII." dans: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 40008-17, (2001) (PubMed).

Tang, Zhang, Low, Wong, Horstmann, Hong: "Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport." dans: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13597-604, (2000) (PubMed).

Détails sur SEC31A

Antigène: SEC31A (SEC31 Homolog A (SEC31A))

Autre désignation: Sec31A

Sujet: Eukaryotic protein trafficking involves packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their contents into an acceptor compartment. Vesicles that bud from the Golgi cisternae and from the ER contain a non-clathrin based coat. This coat is an oligomeric complex whose subunits are termed COPs (coatomer proteins). COPI is the golgi- and endosome-associated COP complex, while COPII is the ER-associated coat complex. In yeast, the COPII complex has a cargo binding subcomplex that includes Sar1p, Sec23p, and Sec24p, which recruits Sec13p and Sec31p leading to vesicle formation. The human homologues of Sec31p are Sec31A and Sec31B. Sec31A has 40% identity with Sec31B, and contains an N-terminal WD-40 domain and a C-terminal proline-rich region. Sec31A mRNA is widely expressed, while Sec31B is found only in testis and thymus. In HeLa, Sec31A localizes to vesicular structures in the perinuclear region of the cell, and co-localizes with the COPII component Sec13. Antibodies against Sec31A inhibit ER to Golgi transport of vesicular stomatitis G protein. Thus, Sec31 may be an important COPII component involved in ER to Golgi transport, as well as a marker for COPII vesicles.

Poids moléculaire: 148 kDa

Pathways: ER-Nucleus Signaling