RUVBL2 anticorps (AA 180-228)

N° du produit ABIN968851

Cet anticorps Souris Monoclonal détecte spécifiquement RUVBL2 dans WB et IF. Il présente une réactivité envers Humain, Souris, Rat, Chien et Poulet et a été mentionné dans 3+ publications.

Aperçu rapide pour RUVBL2 anticorps (AA 180-228) (ABIN968851)

Antigène: RUVBL2 (RuvB-Like 2 (E. Coli) (RUVBL2))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RUVBL2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 42-TIP49b

Détail du produit anti-RUVBL2 anticorps

Épitope: AA 180-228

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Reptin 52/TIF-496 aa. 180-228

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-RUVBL2 (ABIN968851)

Cho, Bhoumik, Broday, Ivanov, Rosenstein, Ronai: "TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage." dans: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8398-413, (2001) (PubMed).

Bauer, Chauvet, Huber, Usseglio, Rothbächer, Aragnol, Kemler, Pradel: "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity." dans: The EMBO journal, Vol. 19, Issue 22, pp. 6121-30, (2000) (PubMed).

Kanemaki, Kurokawa, Matsu-ura, Makino, Masani, Okazaki, Morishita, Tamura: "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a." dans: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22437-44, (1999) (PubMed).

Détails sur RUVBL2

Antigène: RUVBL2 (RuvB-Like 2 (E. Coli) (RUVBL2))

Autre désignation: TIP49b

Sujet: Processes involving DNA transcription, replication and repair are necessary in maintaining normal cell function. For each of these processes, the double stranded NA must first be unwound. This is achieved in an ATP-dependent manner by enzymes termed DNA helicases. Reptin52/TIP49b is a DNA helicase that directly interacts with Pontin52/TIP49a. Reptin52/TIP49b and Pontin52/TIP49a belong to a family of highly conserved proteins that share homology with the bacterial protein RuvB, a helicase involved in double-strand break repair and homologous recombination. Both are TATA binding protein (TBP) interacting proteins that bind to beta-Catenin and are among the 12 polypeptides that comprise the INO80 chromatin-remodeling complex. It has also been demonstrated that Reptin52/TIP49b and Pontin52/TIP49a can be found in a complex with the proto- ncogene c-myc. Reptin52/TIP49b and Pontin52/TIP49a have antagonistic roles as Reptin/TIP49b represses gene activation mediated by beta-Catenin and c-myc, whereas Pontin52/TIP49a stimulates gene activation. Thus, Reptin52/TIP49b and Pontin52/TIP49a are transcriptional co-factors that work together to help control multiple pathways.

Poids moléculaire: 52 kDa

Pathways: Telomere Maintenance