SLU7 anticorps (AA 457-570)

N° du produit ABIN968890

Cet anticorps Souris Monoclonal détecte spécifiquement SLU7 dans WB et IF. Il présente une réactivité envers Humain, Souris, Rat, Chien et Poulet et a été mentionné dans 2+ publications.

Aperçu rapide pour SLU7 anticorps (AA 457-570) (ABIN968890)

Antigène: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SLU7 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 35-hSlu7

Détail du produit anti-SLU7 anticorps

Épitope: AA 457-570

Réactivité croisée: Chien, Rat (Rattus), Souris

Aucune reactivité croisée: Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human hSlu7 aa. 457-570

Isotype: IgG2b

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-SLU7 (ABIN968890)

Chua, Reed: "The RNA splicing factor hSlu7 is required for correct 3' splice-site choice." dans: Nature, Vol. 402, Issue 6758, pp. 207-10, (2000) (PubMed).

Chua, Reed: "Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing." dans: Genes & development, Vol. 13, Issue 7, pp. 841-50, (1999) (PubMed).

Détails sur SLU7

Antigène: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Autre désignation: hSlu7

Classe de substances: Influenza Protein

Sujet: Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3' splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), Prp18p (hPrp18) and Slu7 (hSlu7). hSlu7 contains a zinc knuckle motif similar to the yeast Slu7. This domain is present in retroviral nucleocapsid proteins and in several splicing factors. hSlu7 associates with the spliceosome late in the splicing pathway during recognition of the 3' splice site. During step II of 3' splicing, hSlu7 tightly binds to exon 1 in the spliceosome and helps specify attack on the correct adenine-guanine dinucleotide, located 18 to 40 nucleotides downstream of the branch site.

Poids moléculaire: 80 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interactome