OPA1 anticorps (AA 708-830)

N° du produit ABIN968891

L’anticorps Souris Monoclonal anti-OPA1 a été validé pour WB et IF. Il convient pour détecter OPA1 dans des échantillons de Humain, Rat, Souris, Poulet et Chien. Il y a 3+ publications disponibles.

Aperçu rapide pour OPA1 anticorps (AA 708-830) (ABIN968891)

Antigène: OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))

Reactivité: Humain, Rat, Souris, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp OPA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 18-OPA1

Détail du produit anti-OPA1 anticorps

Épitope: AA 708-830

Réactivité croisée: Chien, Rat (Rattus), Souris, Poulet

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human OPA1 aa. 708-830

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-OPA1 (ABIN968891)

Misaka, Miyashita, Kubo: "Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology." dans: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15834-42, (2002) (PubMed).

Alexander, Votruba, Pesch, Thiselton, Mayer, Moore, Rodriguez, Kellner, Leo-Kottler, Auburger, Bhattacharya, Wissinger: "OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28." dans: Nature genetics, Vol. 26, Issue 2, pp. 211-5, (2000) (PubMed).

Delettre, Lenaers, Griffoin, Gigarel, Lorenzo, Belenguer, Pelloquin, Grosgeorge, Turc-Carel, Perret, Astarie-Dequeker, Lasquellec, Arnaud, Ducommun, Kaplan, Hamel: "Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy." dans: Nature genetics, Vol. 26, Issue 2, pp. 207-10, (2000) (PubMed).

Détails sur OPA1

Antigène: OPA1 (Optic Atrophy 1 (Autosomal Dominant) (OPA1))

Autre désignation: OPA1

Sujet: Three major GTP-binding protein families include trimeric and low molecular weight G-proteins, as well as a family of large proteins homologous to dynamin. The dynamin family contains proteins with diverse structure and function, but highly homologous N-terminal GTPase domains. A subgroup of the dynamin G-protein-binding family includes the mitochondrial proteins Drp1/Dnm1, Mgm1, and OPA1. The latter protein is mutated in dominant optic atrophy, a disease that involves loss of visual acuity and atrophy of the optic nerve. OPA1 is expressed in heart, brain, liver, and kidney. The sequence of OPA1 includes an N-terminal region that contains a mitochondrial targeting domain and three GTP-binding motifs. The overexpression of OPA1 in Cos-7 cells shows co-localization with cytochrome c in mitochondria, and leads to alterations in mitochondrial morphology from a characteristic tubuluar shape to a vesicular pattern. Thus, OPA1 may have roles in mitochondrial biogenesis that are critical for normal cell function. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 80-100 kDa

Pathways: Tube Formation