MLH1 anticorps (AA 381-483)

N° du produit ABIN969285

Cet anticorps anti-MLH1 est un anticorps Souris Monoclonal détectant MLH1 dans WB, ELISA, IHC et ICC. Adapté pour Humain et Singe. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour MLH1 anticorps (AA 381-483) (ABIN969285)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 4C9C7

Détail du produit anti-MLH1 anticorps

Épitope: AA 381-483

Fonction: MLH1 Antibody

Purification: Ascitic fluid

Immunogène: Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-MLH1 (ABIN969285)

Geary, Sasieni, Houlston, Izatt, Eeles, Payne, Fisher, Hodgson: "Gene-related cancer spectrum in families with hereditary non-polyposis colorectal cancer (HNPCC)." dans: Familial cancer, Vol. 7, Issue 2, pp. 163-72, (2008) (PubMed).

Latta, Mohan, Issekutz: "CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis." dans: Immunology, Vol. 121, Issue 4, pp. 555-64, (2007) (PubMed).

Zeng, Kinsella: "A novel role for DNA mismatch repair and the autophagic processing of chemotherapy drugs in human tumor cells." dans: Autophagy, Vol. 3, Issue 4, pp. 368-70, (2007) (PubMed).

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1

Sujet: DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.

Poids moléculaire: 85 kDa

ID gène: 4292

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response