PLK1 anticorps (AA 331-508)

N° du produit ABIN969580

Cet anticorps Souris Monoclonal détecte spécifiquement PLK1 dans ELISA et ICC. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour PLK1 anticorps (AA 331-508) (ABIN969580)

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PLK1 est non-conjugé

Application: ELISA, Immunocytochemistry (ICC)

Clone: 1D1

Détail du produit anti-PLK1 anticorps

Épitope: AA 331-508

Fonction: PLK1 Antibody

Purification: Purified antibody

Immunogène: Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

ICC: 1/50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-PLK1 (ABIN969580)

Lee, Rhee: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." dans: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, (2011) (PubMed).

Li, Zhou, Ye, Cheng, Zhou, Lu, Xie: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." dans: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, (2011) (PubMed).

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1

Sujet: PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation.

Poids moléculaire: 68 kDa

ID gène: 5347

UniProt: P53350

Pathways: Cycle Cellulaire, M Phase