CDK2 anticorps (C-Term)

N° du produit ABIN97130

N° du produit (Fournisseur): 100-401-161

Cet anticorps anti-CDK2 est un anticorps Lapin Polyclonal détectant CDK2 dans WB, IHC, ELISA, IP et FM. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CDK2 anticorps (C-Term) (ABIN97130)

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Détail du produit anti-CDK2 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 100-401-161

Fournisseur: Rockland

Fonction: cdk2 Antibody

Réactivité croisée (Details): Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue.

Attributs du produit: Synonyms: rabbit anti-Cdk2 Antibody, Cdc2 related protein kinase antibody, Cdk 2 antibody, CDKN2 antibody, Cell division kinase 2 antibody, Cell division protein kinase 2 antibody, rabbit anti-Cyclin dependent kinase 2 antibody, p33 protein kinase antibody, cdk

Purification: Anti-cdk2 antibody was prepared from monospecific antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-cdk2 antibody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-cdk2 antibody has been tested in western blot and immunohistochemistry and is suitable for IF and other antibody based assays.  Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry.  Optimal titers for other applications should be determined by the researcher.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CDK2

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Autre désignation: CDK2

Sujet: Background: Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

ID gène: 1017

UniProt: P24941

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA