Cyclin B1 anticorps

N° du produit ABIN97948

N° du produit (Fournisseur): 100-401-152

Cet anticorps Lapin Polyclonal détecte spécifiquement Cyclin B1 dans WB, IHC, ELISA et IP. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour Cyclin B1 anticorps (ABIN97948)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Cyclin B1 anticorps

N° du produit (Fournisseur): 100-401-152

Fournisseur: Rockland

Fonction: Cyclin B1 Antibody

Réactivité croisée (Details): Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.

Attributs du produit: Synonyms: rabbit anti-cyclin B1 antibody, G2/mitotic-specific cyclin-B1

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500

Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

Western Blot Dilution: 1:500 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 80 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-Cyclin B1 (ABIN97948)

Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." dans: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, (2014) (PubMed).

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: CCNB1

Sujet: Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

ID gène: 891, 14327896

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase