(2 références)
N° du produit ABIN1379871
Aperçu rapide pour Fixation Buffer (ABIN1379871)
Application
Intracellular Flow Cytometry (ICFC)
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Marque
- BD Cytofix™
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Attributs du produit
- BD Cytofix™ Fixation Buffer is comprised of a neutral pH-buffered saline (i.e., Dulbecco's Phosphate-Buffered Saline) that contains 4% w/v paraformaldehyde. This fixation buffer is intended to preserve human and rodent lymphoid cells for the subsequent immunofluorescent staining of intracellular cytokines. BD Cytofix can also be used to preserve the light-scattering characteristics and fluorescence intensities of human and rodent hematopoietic cells that have been stained by immunofluorescence for subsequent flow cytometric analysis.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS Buffer pH 7.5BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C
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Stockage commentaire
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." dans: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
: "Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis." dans: Journal of immunological methods, Vol. 47, Issue 1, pp. 25-30, (1982) (PubMed).
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: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." dans: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
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